=COUNTIF($C$1:$C$2000,C1)=1
Note that the first two cell references in the forumula are absolute (by using the $). The cell range of $C$1:$C$2000 means that those are the cells whose values are checked against your current entry. In my example, I'll never have more than 2000 entries, so this will work just fine. For your use, be sure to enter a number high enough to cover the number of rows you'll have.